|
KMID : 0525720000050030160
|
|
Journal of Chitin and Chitosan
2000 Volume.5 No. 3 p.160 ~ p.164
|
|
|
Hydrolysis of Chitosan by Gizzards of Broiler ( Gallus gallus L. )
|
|
한범구/Han BK
조성준/문종국/김새봄/조도현/Cho SJ/Moon JK/Kim SB/Jo DH
|
|
|
Abstract
|
|
|
|
Low-cost enzyme sources are requested for the production of chitosan oligomers, which were recently spotlighted as functional oligosaccharides. Gizzard tissue of broiler and its chyme are selected for the production of chitosan oligomers, and chitosanolytic activities were investigated. Chitosanolytic activity of chyme was about two times higher than that of gizzard tissue. Repeated freezing and thawing did not affect the activity of crude enzyme preparations. Optimal pH for the gizzard and the chyme were 3.0 and 2.8, respectively. Optimal temperature of the crude enzymes ranged from 60℃ to 70℃ and their activities were maintained at 70% level at 80℃. At 50℃ for three days no decrease in their activity was observed. After 24 hour incubation, gizzard and chyme produced 40 yg oligomers/mg-protein and 80 yg oligomers/mg-protein, respectively. Under optimal conditions, 62% of total substrate were made water-soluble by the gizzard. Trimer of glucosamine was identified as its main reaction product.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|